Coding

Part:BBa_K1988010:Design

Designed by: Bowman Clark   Group: iGEM16_Purdue   (2016-10-14)


PPGK ATPi: Polyphosphate ATP Independent Glucokinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 135
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E. coli K-12 using IDT Codon Optimizer


Source

Genetic sequence from 'Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus Phosphovorus' by Kawakoshi, et. al.

References